Diseases caused by viruses and chlamydia Avian influenza (Fowl plague, highly pathogenic avian influenza (HPAI))
Avian influenza is a viral disease of several avian species in various parts of the world. The disease can range from asymptomatic and mild to hyperacute and fatal. Avian influenza occurs infrequently in humans. It is seen as an occupational hazard, primarily to those associated with varied activities in the poultry industry; employees in abattoirs, vaccinators, laboratory staff and other personnel. In most cases the clinical picture is that of conjunctivitis with rare systemic reactions. Avian influenza is reportable disease in many countries. It has to be confirmed by virus isolation.
Transmission : Secretions from infected birds, by wild birds and contaminated feed, equipment and people. Seabirds and migratory waterfowl comprise the main reservoir for avian influenza virus.
Antemortem findings:
The incubation period varies from a few hours to about seven days.
The morbidity and mortality rates can reach 100 % in cases of highly pathogenic strain of the viruses.
Marked depression, loss of appetite and watery diarrhoea
Coughing, sneezing, rales, excessive lacrimation
Drop in egg production in layers
The conjunctiva is congested and swollen, and occasionally haemorrhagic.
Swollen combs with cyanotic tips and haemorrhagic surface
Edematous wattles (Fig. 187) and edema around the eyes, head and neck
Ruffled feathers and dark red skin (Fig. 188)
Diffuse haemorrhages between the hocks and feet
Blood in the cloaca
Some birds may recover, even after being severely affected.
Postmortem findings :
Birds that die with the peracute form of AI may show no significant gross lesions
Dehydration
In highly pathogenic influenza virus, fibrinous exudate is found in airsacs, oviduct, peritoneum and pericardial sacs.
Mild to moderate infection
Inflammation of conjunctivae, trachea and airsacs
Pronounced congestion of the musculature
Ovarian regression in laying birds
Edema of the head with congestion, haemorrhages and cyanosis of the combs, wattles and sinuses
Vesicles and ulceration of the comb
Petechial and ecchymotic haemorrhages in abdominal fat, various serosal and mucosal surfaces, heart, gizzards, proventriculus and small intestine (Fig. 189)
The feet often appear edematous with haemorrhages. Red discoloration of the shanks is also noted.
Judgement : Carcasses affected with avian influenza in any form should be condemned.
Differential diagnosis : Fowl cholera, chlamydiosis, mycoplasmosis, velogenic viscerotropic Newcastle disease
Fig. 187: AI. Edematous, cyanotic comb and wattles of a chicken.
Fig. 188: Bloody cloaca and dark coloured skin of a chicken that died of AI.
Fig. 189: AI. Haemorrhage in the small intestine, between two dark coloured ceca.
Newcastle Disease (NCD)
Velogenic Viscerotropic Newcastle disease (VVND) or Asiatic Newcastle disease (AND)
NCD is in its chronic form an infection of domestic fowl with symptoms such as rejection of food, listlessness, abnormal breathing, discharge from eyes and greenish diarrhoea. Mortality in chicken is 50 – 80 %, but in adults much lower due to available vaccination. VVND is an acute, fatal infection of birds of all ages with predominant haemorrhagic lesions of the gastrointestinal tract, severe depression, and death prior to clinical manifestations. This disease is caused by the most virulent strain of the Newcastle disease virus. The virus of VVND is very resistant and remains viable at extreme pH and temperature ranges, and may remain viable in the bone marrow of poultry carcasses for weeks.
Transmission : Transmission is by direct contact, fomites, and by aerosols through coughing, gasping and respiratory fluids. The virus has a wind borne potential for spread creating quite a challenge for control and prevention. Faeces and insect and rodent vectors are also involved in the transmission.
Antemortem findings :
The incubation period varies from 2 – 15 days.
Depression and loss of appetite
Sudden death
Edema of the head. Swelling of the lower eyelid, often accompanied by conjunctivitis (Fig. 190).
Dark ring around the eye (black eye)
Excessive fluids from the respiratory tract
Paralysed wings and twisting of the head and neck (torticollis)
Postmortem findings :
Acute form
Peracute deaths will often show no discernible lesions in some of the first birds dying in an outbreak.
The oesophagus shows haemorrhage and erosions.
Edema of the head and neck
The mucosa of the trachea is frequently haemorrhagic (Fig. 191 upper).
Haemorrhages are throughout the gastrointestinal tract with a tendency to ulcerate and become necrotic as the disease progresses.
In the intestine there is generally an inflammatory response and marked involvement of the caecal tonsils and Peyer's patches (Fig. 191 middle).
The mucosal lining of the proventriculus is a frequent site of haemorrhage, especially at the junction between the oesophagus and proventriculus (Fig. 191 bottom).
Edematous or haemorrhagic ovaries
In hens that have survived the disease, there is a tendency to lay misshapen eggs or develop egg yolk peritonitis.
Chronic form
Catarrhal inflammation of the respiratory system
Edema in surrounding connective tissue
Judgement : Birds with VVND or NCD should not be admitted to the abattoir. If disease is suspected laboratory confirmation should be obtained. If confirmed, carcass is condemned and premises with equipment should be disinfected. In case that laboratory confirmation is not possible, suspected carcasses should be also condemned. In some countries compensation is paid for condemned birds.
Differential diagnosis : VVND and NCD must be differentiated from the following diseases: Infectious bronchitis, laryngotracheitis, fowl cholera, highly pathogenic avian influenza (HPAI), fowl pox (diphtheritic form), psittacosis, acute Mycoplasma gallisepticum infection, avian encephalomyelitis, vitamin E deficiency, Marek's disease and Pacheco's disease in parrots
Fig. 190: New Castle disease (NCD). Swelling of the lower eyelid and conjunctivitis.
Fig. 191: NCD. Acute form: Haemorrhage in the mucosa of the trachea (upper), large intestine, particularly caecal tonsils (middle), proventriculus (bottom) and gizzard.
Infectious bronchitis (IB)
Infectious bronchitis is an acute, highly contagious viral disease of chickens, manifested by respiratory signs, renal disease and a significant drop in egg production.
Transmission : Airborne transmission in the direction of prevailing wind. The spread of infection is rapid in a flock. Some birds become carriers and shedders of the virus through secretions and discharges for many months after the infection. IB virus persists in contaminated chicken houses for approximately four weeks.
Antemortem findings:
Indifference and depression
Sneezing, gasping and coughing
Nasal discharge
Abnormal respiratory sounds (rales)
Weakness and huddling near the light source
Reduced egg production in laying birds. Low egg quality and soft egg shells are noted.
Mortality due to kidney disease caused by the nephrotropic strain of the IB virus.
Inflammation of the air sacs may be a complication of IB.
Postmortem findings:
Serous, catarrhal and caseous exudate in the upper respiratory tract including nasal passages, trachea, sinuses and bronchi
Cloudy airsacs
Abdominal airsacs may contain yellow caseous exudate (Fig. 192).
Occasionally swollen and pale kidneys containing urolith deposits (uric acid crystals) (Fig. 193)
Yolk material or fully formed egg in the abdominal cavity in layer hens
Small cystic oviducts
Judgement: Affected birds are treated as suspects on antemortem inspection. A carcass showing acute signs of clinical disease accompanied with emaciation is condemned. A carcass in good flesh and without systemic changes is approved. The affected parts are condemned.
Differential diagnosis: Newcastle disease, laryngotracheitis (LT) and infectious coryza. Laryngotracheitis spreads slowly in a flock although respiratory signs are more severe than in infectious bronchitis. LT is not seen in young chicken.
Fig. 192: IB. Abdominal airsac containing yellowish caseous exudate.
Fig. 193: IB. Swollen kidneys and ureters containing urolith deposits (uric acid crystals).
Laryngotracheitis (LT)
LT is an acute viral disease of chicken characterized by difficult breathing, gasping and coughing up of bloody exudate.
Transmission: Virus entry in LT is via the respiratory route and the intraocular route. Oral infection may also occur. The transmission from acutely infected birds is more common than from recovered or vaccinated birds. The latter may shed the virus for a prolonged period of time. Mechanical transmission via fomites is another possibility.
Antemortem findings:
Incubation 6 – 12 days following natural exposure
High morbidity and moderate mortality (10 – 20 %)
Difficult breathing and coughing (Fig. 194)
Loud gasping or wheezing sounds
In mild form of LT, lacrimation, nasal discharge, swelling of conjunctivae and sinuses and reduced egg production.
Most chicken usually recover in 10 –14 days and up to 4 weeks in severe cases.
Postmortem findings:
Inflammation of the larynx and trachea leading to necrosis and haemorrhage of mucosa
Extension of infection to the bronchi, lungs and airsacs
Death due to pseudomembranes or cheesy plugs in the trachea (Fig. 195)
Judgement: Mild form of disease and recovered birds may have a favourable judgement if carcass is in good flesh. If an acute condition is associated with general systemic changes, the carcass is condemned.
Differential diagnosis : Newcastle disease, Infectious bronchitis and infectious coryza
Fig. 194: LT. Difficult breathing and coughing.
Fig. 195: LT. Inflamed trachea containing cheesy plugs.
Fowl Pox (FP, Pox, Avian Pox)
Fowl Pox is a viral disease of chicken, turkeys and other birds distinguished by cutaneous lesions on the head, neck, legs and feet. It has a worldwide distribution and affects birds of all age groups, except the recently hatched.
Transmission : The virus is present in lesions and in desquamated scabs. It is resistant to environmental factors and persists in the environment for many months. It usually infects birds through minor abrasions. Mechanical transmission occurs by cannibalism. Some mosquitoes can transmit the virus from infected to uninfected birds. The virus can be also transmitted by injury to the skin.
Antemortem findings: Two forms of lesions are recognized, - the cutaneous (dry form) and the diphtheric (wet form)
Cutaneous form
Low mortality
Lack of flock vigour and weight loss
A mild to moderate loss in egg production
Scabby lesions on the head, neck and unfeathered parts of the skin (Fig. 196)
Diphtheric form
Mortality low to moderate
Difficult breathing
Nasal and ocular discharge
Postmortem findings: The following stages of the pox lesionspapules, vesicles and pustules may be observed.
Cutaneous lesions
Papules are light coloured nodules.
Vesicles and pustules are raised and commonly yellow.
Diphtheric lesions
Buff to yellow plaques on mucous membranes in the mouth (Fig. 197), oesophagus and upper respiratory tract
Occlusion of trachea, and death due to asphyxiation
Histopathology shows characteristic intracytoplasmic inclusion bodies (Bollinger bodies) in the infected epithelium.
Judgement : Carcass affected with fowl pox is condemned if progressive generalized lesions in a bird are accompanied with emaciation. Fowls with localized lesions and recovered birds are approved after the removal of scales.
Differential diagnosis : Pantothenic acid and biotin deficiency, vitamin E deficiency, infectious laryngotracheitis and other respiratory diseases in poultry, injuries caused by external parasites and cannibalism.
Fig. 196: FP. Cutaneous form (dry pox). Face lesions in young turkey.
Fig. 197: FP. Diphtheric form (wet pox). Caseous lesions in the mouth and throat of a chicken.
Avian leucosis complex
Avian leucosis complex occurs in four separate disease entities:
Leucosis-sarcoma group (Lymphoid leukosis)
Marek's disease (MD)
Reticuloendotheliosis group (REV)
Lymphoproliferative agent of turkey
Transmission : L/S virus is transmitted by egg in vertical transmission and by shedders in horizontal transmission (chicken to chicken). Tumour lesions may or may not develop. Developed tumours can be differentiated by the bird's age on necropsy, histology and impression smears. In horizontal transmission, chicken which contract the virus after hatching develop antibodies; some will remain shedders, some will develop tumours and die, and others will overcome the infection. Infection from flock to flock is unlikely as the virus does not survive a long time in the environment. In many chickens the virus may be also in a latent state.
(A) Lymphoid leucosis (big liver disease, visceral lymphomatosis)
This disease is being studied because of its economic significance, and also as an experimental model of cancer. Lymphoid leucosis is a B cell tumour which starts in the bursa and, before sexual maturity, may spread to other organs. Mature birds are more affected than young birds. Male birds are also affected in lesser numbers than female due to the earlier regression of bursa in male birds.
Antemortem findings :
The disease occurs in 14 – 30 weeks old birds.
Pale, shrivelled comb and loss of appetite
Dehydration and emaciation
Diarrhoea, green scant faeces
Enlarged liver, bursa of Fabricius and kidneys
Distended abdomen due to enlarged liver
Reduced egg production
Postmortem findings :
Grey tumour lesions4 in the liver (Fig. 198), spleen and bursa
Other organs such as lung, heart, proventriculus, gonads, bone marrow and mesentery are sometimes affected.
Ecchymotic haemorrhages around the skin follicles of the wing
Judgement : The carcass of a bird affected with lymphoid leucosis is condemned. The condemned material may be used for animal food.
Differential diagnosis : Between lymphoid leucosis and Marek's disease is shown in Table 3.
Fig. 198: Avian leucosis complex. Diffuse nodular lesions in the liver, spleen, intestine and heart.
4 Tumour lesions are nodular, miliary or diffuse. Nodules are approximately 0.5 cm – 5 cm in diameter, miliary lesions are 2 mm in diameter and diffuse lesions are noted as a uniform enlargement of the organs.
(B) Marek's Disease (Polyneuritis, Fowl paralysis, Neurolymphomatosis gallinarum)
Marek's disease is caused by the herpes virus (DNA).
Transmission : It is spread by airborne infection involving follicle cells called chicken dander. Transmission of the virus is horizontal. At room temperature the virus of Marek's disease remains viable for 16 weeks and in litter for 6 weeks. Birds are most susceptible to the infection during the first few weeks of life. Infected birds will start to shed the virus in the second or third week after infection and will continue to do so throughout their life, although they do develop antibodies against the virus.
Marek's disease is commonly associated with coccidiosis in the field. This is probably due to lack of immunity against coccidiosis in birds affected with Marek's. There are 6 types of Marek's disease.
Peracute in 3–5 weeks old chicks; manifested with sudden death
Anaemia in 3–6 weeks old chicken
Classical Marek's disease (range paralysis) showing paralysis of two wings and legs due to peripheral nerve involvement. The nerves are 2–3 times their normal size. Central nerve involvement may also occur.
Acute Marek's disease occurs mainly in 6–12 weeks old birds. It is manifested with tumours in the liver, spleen, kidneys, brain, spinal cord and dorsal root ganglia and with sudden death. The heart, lungs, gonads and muscles may also be involved.
Skin leucosis. Noted in broiler chicken on postmortem examination in abattoirs as enlargement of feather follicles and associated lymphoid infiltrations (Fig. 199).
Transient paralysis in 12–18 weeks old pullets become paralysed and may recover with 24 hours if moved to a quiet place.
Fig. 199: Avian leucosis complex. Neoplastic lesions of Marek's disease in the leg.
Postmortem findings :
Skin neoplasia
Enlarged spleen
Diffuse proliferation of white pulp
Judgement : Carcass of a chicken affected with Marek's disease is condemned in the extensive cutaneous form and in the visceral form. Localized skin lesions require the condemnation of the affected portions, the rest of the carcass may be approved. The condemned material may be used for animal food.
Differential diagnosis (for skin lesions) : Lymphoid leucosis (see Table 7), erythema, dermatitis, pigmentation and normal large follicles. If the central nervous system is affected the lesions of Marek's disease should be distinguished from those of Newcastle disease and of avian encephalomyelitis. In immunized birds for Marek's disease, the inflammation of the feather follicles (folliculitis) may be caused by the Marek's disease virus. In both cases follicular enlargement is noted; however, the lesion may differ in colour. In immunized birds the lesions of folliculitis are yellowish-white, whereas with the Marek's virus, lesions are blue-grey in colour with a pinkish appearance due to petechial haemorrhage.
Table 3: Differential diagnosis between lymphoid leucosis and Marek's diseaseFeatureLymphoid leucosisMarek's diseasesAge of onset16 weeks4–6 weeks or olderSymptomsAbsentFrequently paralysis or paresisIncidenceSeldom above 5 %Usually above 5 %Gross Lesions Peripheral nerve enlargementAbsentUsually presentBursa of FabriciusNodular tumoursDiffuse enlargement or atrophySkin, muscle orUsually absentMay be presentproventriculus tumours Microscopic Lesions Peripheral nerve infiltrationAbsentPresentCuffing in white matter ofAbsentPresentcerebellum Tumour in the liverFocal or diffuseFrequently perivascularBursa of FabriciusIntra-follicular tumourInter-follicular tumours or atrophyFollicular patterns of lymphoid cells infiltration in the skinAbsentPresentCytologyUniform lymphoblastsPleomorphic mature and immature cells including lymphoblast, small medium and large lymphocytes and reticulum cells.
Ornithosis (Psittacosis, Avian chlamydiosis)
Ornithosis is an acute or chronic disease of turkeys, ducks, chicken, pheasants and pigeons. It is caused by Chlamydia psittaci. In psittacine birds such as parrots, parakeets, cockatoos, macaws etc. and humans, this disease is called psittacosis.
Transmission : Wild carrier birds and cage birds transmit Chlamydia to their nestling which may survive and become carriers. Carrier birds shed Chlamydia in their secretions and excretions. Chlamydia present in faecal dust may be inhaled or ingested. Pigeons are suspected of being disseminators of infection.
Antemortem findings :
Mild cases may be unobserved or show mild respiratory signs and diarrhoea
In turkeys
Depression and weakness
Reduced weight
Nasal discharge
Green yellow diarrhoea
Watery diarrhoea in ducks, geese
In pigeons
Unilateral or bilateral conjunctivitis
Depression and loss of appetite
Abnormal respiratory sounds (rales) and watery diarrhoea
Postmortem findings :
Inflammation of the lungs, airsacs, liver, heart, spleen, kidneys and peritoneum
In turkeys
Wasting
Vascular congestion
Fibrinous inflammation of pericardium, airsacs, lungs
Congestion of the lungs and an enlarged congested spleen
In pigeons
Swollen eyelids and conjunctivitis
In cage birds enlarged spleen and liver, inflammation of the airsacs and pericardium and intestinal congestion.
Judgement : If the disease is suspected on antemortem, birds are treated as suspects and samples should be shipped to the Laboratory. All reasonable precautions should be taken to avoid risk of human contact. If the disease is suspected on postmortem, delayed slaughter of the birds from the same source should be required. Samples are also forwarded to the diagnostic laboratory. Positive laboratory finding necessitate condemnation of the bird or carcass. If the disease is not confirmed the carcass may be approved if otherwise wholesome.
Differential diagnosis : Mycoplasma gallisepticum infection, pasteurellosis, salmonellosis (pullorum disease; bacillary white diarrhoea(BWD))
Diseases caused by bacteria
Salmonellosis
Salmonellosis is responsible for significant losses to the poultry industry. Salmonella infections in this Manual include pullorum disease, fowl typhoid, arizona infection and paratyphoid. Pullorum disease occurs in chicken and turkeys and is caused by Salmonella pullorum. The greatest losses are in chicken less than 4 weeks old.
Antemortem findings (in young chicks) :
Anorexia
Diarrhoea
Pasting of vent
Stunted, unthrifty
Poor feathering
Pale shrunken comb and wattles
Postmortem findings :
Multiple grey nodules in the heart (Fig. 200), lungs, liver, spleen, peritoneum, the gizzard, intestine and pancreas of a poult
Abnormal ovary showing discoloured follicles in an adult bird
Rarely peritonitis, ascites or oviduct impaction
Swelling of the tibiotarsal joint
Fig. 200: Pullorum disease. Multiple grey nodules in the heart.
Judgement : Carcass and viscera affected with pullorum disease are condemned.
Differential diagnosis : Liver and heart lesions should be differentiated from infections due to other salmonellae and from campylobacteriosis, colibacillosis and omphalitis. Nervous lesions should be distinguished from nervous signs observed in Newcastle disease. Respiratory tract lesions should be differentiated from aspergillosis and joint lesions with synovitis and bursitis caused by other bacteria or viruses.
Fowl typhoid
Fowl typhoid is an infectious disease in chicken and turkeys and rarely in other poultry, game birds and wild birds. The causative agent is Salmonella gallinarum. It is seen more often in adult birds. Antemortem signs of fowl typhoid and pullorum disease are similar in birds.
Postmortem findings :
Enlarged, bronzed liver and enlarged spleen in a turkey (Fig. 201).
Enlarged kidneys
Pale cadaver
Inflammation of the anterior part of the intestine
Judgement : Carcass and viscera affected with fowl typhoid arecondemned.
Differential diagnosis : see pullorum disease
Fig. 201: Fowl typhoid. Enlarged, bronzed to the mahogany colour liver and enlarged, mottled and brittle spleen in a turkey Diseased liver and spleen are shown in contrast to the normal ones at left.
Paratyphoid infection
Paratyphoid infection is an acute and chronic infection of poultry and mammals. Ten to twelve species of Salmonella are associated with most outbreaks in poults. The most commonly involved is S. typhimurium in birds less than one month old.
Antemortem findings :
Drooping wings
Shivering and huddling near a heat source
Muscular incoordination and trembling
Postmortem findings :
Nodular lesions in the pancreas
Button type lesion in the intestine (Fig. 202)
Enteritis
Dehydration
Unabsorbed yolk material and omphalitis
Less frequent are joint infections
Blindness
Swollen eyelids
Judgement : Carcass and viscera affected with paratyphoid infection are condemned.
Differential diagnosis : see pullorum disease
Fig. 202: Paratyphoid infection. Button type lesion in the intestine.
Arizona infection (Arizoonosis, Paracolon infection)
Arizoonosis occurs in young turkey poults. It is caused by Arizona hinshawii (Syn. Salmonella arizona). Arizona infection is egg transmitted.
Antemortem findings :
Listlessness and trembling
Pasting of vent area with faeces
Huddling near a light source
Twisted head and neck
Cloudiness and enlargement of the eye causing blindness
Antemortem findings : Antemortem findings in young birds are similar to those of paratyphoid.
Postmortem findings :
Enlarged yellow liver
Congestion of the duodenum
Unabsorbed yolk material
Cheesy plugs in the intestine or caecum
Inflammation of the oviduct and peritoneum
Eye lesions
Purulent exudate in the brain
Judgement : Carcass and viscera affected with arizoonosis arecondemned.
Differential diagnosis : The causative organism must be isolated and identified for differentiation from salmonellosis.
Fowl cholera (Pasteurellosis)
Fowl cholera is an infectious disease affecting almost all classes of fowl and other poultry. The disease is more prevalent in turkeys than in chicken. It occurs more frequently in stressed birds associated with parasitism, malnutrition, poor sanitation and other conditions. Fowl cholera is caused by Pasteurella multocida. This organism is easily destroyed by sunlight, heat, drying and most of the disinfectants. However, it will survive several days of storage or transportation in a humid environment. It persists for months in decaying carcasses and in moist soil. The agent is frequently carried in the oral cavity of wild and domestic animals.
Transmission : If birds are bitten by infected animals such as rodents and carnivores, the disease could be disseminated in the flock. Contaminated feed, water, soil and equipment are also considered as potential factors in the spreading of the disease.
Antemortem findings :
Acute septicemic form
High morbidity and mortality and sudden death
Dead birds may be found on dropping boards or in nests.
Depressed, cyanotic and loss of appetite
Nasal and oral discharge
Greenish diarrhoea
The comb may be swollen and discoloured.
Emaciated
Chronic fowl cholera
Swelling of wattles, sinuses, joints, foot pad and tendon sheaths
Cheesy exudate in the conjunctival sac
Twisting of the head and neck may be observed in some birds.
Middle ear infection is rare but occurs when the bacterial agent reaches the middle ear through the nasal cavity.
The bird may lose its sense of balance with the head and neck twisted to one side.
If both ears are infected, the bird's head and neck are pulled back over the body and between the legs.
Postmortem findings : In the very acute stages, lesions may be lacking.
Caseous exudate in wattles, sinuses (Fig. 203), the nasal turbinates, middle ear, joints or tendon sheaths.
Petechial and ecchymotic haemorrhages on the heart, serous and mucous membranes, on the gizzard and abdominal fat.
The liver is swollen and is streaked with white areas and associated small grey areas of necrosis (corn meal liver, Fig. 204).
Free yolk in the peritoneal cavity in breeder hens and layers.
Acute oophoritis and peritonitis are often seen.
The lining of the upper intestine is reddened and gut content is slimy.
In chronic cases
Darkened breast muscle is frequently noted and haemorrhagic lesions are often missing.
Judgement : Localized lesions of pasteurellosis such as infection of wattles, joints or tendon sheaths require the condemnation of the affected parts; the rest of the carcass is approved. Septicemic carcasses should be condemned.
Differential diagnosis : Acute colibacillosis and erysipelas in turkeys, salmonellosis, tuberculosis, listeriosis. Pasteurellosis is differentiated from septicemic and viremic diseases by culture of P. multocida. Closely related bacteria such as S. gallinarum, P. haemolytica and others may cause a cholera like disease or they may complicate other diseases.
Public health significance : Fowl cholera, although known for 200 years is still a poorly controlled disease in birds. Humans may become infected with this disease, and then they may also infect poultry with exudates from the nose and mouth.
Fig. 203: Fowl cholera. Cheesy exudate in the Infraorbital Sinus.
Fig. 204: Fowl cholera. Small areas of necrosis in the liver (corn meal liver)
Tuberculosis
Tuberculosis is a chronic granulomatous disease of poultry caused by Mycobacterium avium. It is usually found in older chicken which are kept beyond the laying season. Other poultry are also affected.
Antemortem findings :
Depressed and progressive loss of weight leading to emaciation
Pale skin of the face, wattles and comb
Dull, ruffled feathers
Diarrhoea
Unilateral lameness
Postmortem findings :
Irregular greyish yellow or greyish white nodules of different sizes in the liver (Fig. 205), spleen, and intestine
In advanced cases, TB nodules are found in the bone marrow.
Lesions are rarely found in the heart, ovaries, testes and skin.
TB lesions on organ surfaces are easy enucleated from the surrounding tissue. Mineralization is not present in the lesions.
Judgement : A carcass affected with tuberculosis is condemned.
Differential diagnosis (on postmortem) : Aspergillosis, typhoid and paratyphoid, salmonellosis, fowl cholera, campylobacter, colibacillosis, chlamydiosis, histomoniasis and neoplasm. In neoplasm, the cut surface of the lesion is uniform and without necrosis.
Fig. 205: Tuberculosis. Greyish white nodules in the liver
“Air sac disease” and Chronic Respiratory Disease (CRD)
The term “Air sac disease” usually refers to a respiratory syndrome characterized by airsacculitis, perihepatitis and pericarditis in broiler chickens between 4 – 8 weeks of age. Pneumonia is also frequently present. Primary factors associated with the etiology of air sac disease are poor air quality and dust, associated with either viral or mycoplasmal agents. E. coli is usually a secondary invader.
Chronic respiratory disease (CRD) refers to respiratory infection of the upper respiratory tract of chicken caused by Mycoplasma gallisepticum. This agent affects turkeys more severely and causes infectious sinusitis.
Postmortem findings : Postmortem examination of affected chicken reveals inflammation of trachea and frothy exudate in the air sacs. With presence of secondary invaders, inflamed airsacs have a opaque appearance in the early stages of infection (Fig. 206). In the later stages airsacs are thickened and caseous yellow exudate is usually present. Yellow fibrinous deposits on the pericardium (pericarditis) and liver (perihepatitis, Fig. 207) are also observed.
Fig. 206: Chronic Respiratory Disease. Cloudy appearance of the abdominal airsacs in this 7 week old chicken.
Fig. 207: Pericarditis and perihepatitis in an eight week old chicken. E. coli was isolated from both lesions.
Judgement : In poultry inspection, it is of great importance to differentiate between the inflammation of the air sacs (airsacculitis) and peritonitis, as well as pathologic changes in the air sacs and bones. The communication of some air sacs and bones has to be observed during the judgement of carcasses with airsacculitis and bone diseases. Birds affected with airsacculitis are treated as suspects on antemortem examination. On postmortem examination affected parts with localized lesions are condemned and the rest of the carcass is approved if in good condition and no spread of infection into bones is noted. The rational for approving a carcass in good condition with localized lesions of airsacculitis may be supported with frequent negative findings of microorganisms in these lesions. Since an accumulation of the fluid in the airsacs and associated opacity may be early signs of infection, the other birds in the flock must also be examined. Sufficient evidence must be obtained in that these lesions would remain localized. Otherwise the carcass must be condemned. Generalized and extensive lesions of airsacculitis require total condemnation of the carcass. If pericarditis or perihepatitis are the only lesions present on postmortem the carcass may be approved if otherwise wholesome.
Parasitic diseases
Diseases caused by protozoa
Histomoniasis (Enterohepatitis or Blackhead)
Histomoniasis is a protozoal disease of turkeys, chicken and game birds. It often occurs in turkeys run with or after chicken. It is caused by Histomonas meleagridis which is transmitted in the ova of caecal worms (Heterakis gallinarum) and by earth worms. Well developed hepatic and caecal lesions are pathognomonic for histomoniasis.
Antemortem findings :
In young turkeys, mortality and morbidity may reach 100 %.
Indifference and loss of appetite
Depression, drooping wings and closed eyes
Cyanotic (black) head
Yellow sulphur coloured faeces
Blood in faeces
Emaciation in older birds
Postmortem findings :
The ceca are characterized with a thickened wall, ulcerated mucosa and caseous exudate.
If the caecal wall becomes perforated, inflammation of the peritoneum is observed.
The liver lesions consist of circular yellow necrotic tissue surrounded with white rings (Fig. 208).
On microscopy, histomonas are found in the caecal wall or in the liver lesion.
Fig. 208: Histomoniasis. Circular yellow necrotic tissue surrounded with white rings of a turkey liver.
Judgement : The bird is approved if in good flesh. The liver and intestine are condemned. The carcass is condemned if emaciated or associated with septicemia.
Differential diagnosis : Salmonellosis, coccidiosis, aspergillosis and trichomoniasis.
Coccidiosis
Coccidiosis is a major disease problem in commercial poultry and the most common cause of enteritis. It is caused by various species of the protozoan genus Eimeria. At least nine species have been described in chicken. The most important of these are E. acervulina, E. brunetti, E. maxima, E. necatrix and E. tenella.
The identification of different species of coccidia is based on the distribution of the lesion in the intestine, gross and microscopic appearance, and the size and shape of oocysts. Coccidia are species specific which means that coccidia in chicken will not be found in turkeys and vice versa.
Whether the coccidia actually cause illness or death of the bird depends on the species, dose, location of parasitic reproduction and immune status of the bird. Birds affected with Marek's disease and IBD are more susceptible to coccidiosis.
If parasitic reproduction occurs in the epithelium of the intestine, disease follows and if it occurs subendothelially, the birds usually bleed to death in acute cases.
With ingested oocysts moving to the intestinal lining, the life cycle of coccidia begins. Intestinal cells are damaged and in 4 – 8 days, depending on the species, oocysts are shed in the faeces.
Within 12 – 30 hours in a moist environment and in a temperature of 20 – 30°C, the oocysts sporulate and become infective. Birds raised on the range will have more outbreaks of this condition in the spring and summer compared to birds raised in total confinement of barnyards. In the latter, outbreaks can occur at any time.
Coccidiosis causes severe losses in intensive broiler production and in layers, it causes a drop in egg production. This is in spite of great discoveries of many different coccidiostats. Even when the disease is controlled, medication expenses run very high.
Antemortem findings :
Ruffled feathers
Anaemia and dehydration
Retracted head and neck
Bloody diarrhoea
Weight loss
Findings in poults include:
Listlessness and droopiness
Ruffled feathers
Light brown mucoid diarrhoea
Huddling in groups
Judgement : Birds affected with severe coccidiosis associated with emaciation or anaemia are condemned. Otherwise, the carcass is approved, after the condemnation of affected tissue.
Differential diagnosis (on postmortem) : Necrotic enteritis, ulcerative enteritis, salmonellosis, ascariasis, capillariasis, haemorrhagic disease, leucosis, blackhead, bluecomb and haemorrhagic enteritis in turkeys
I. Chicken coccidiosis
(A) E. acervulina
E. acervulina is found in the duodenum and upper jejunum. It is frequent in growing birds and broilers and causes duodenal coccidiosis.
Postmortem findings:
Whitish transverse lesions which may coalesce.
Parasite location in tissue section is epithelial.
(B) E. brunetti
E. brunetti is found in the lower small intestine, rectum and proximal part of ceca.
Postmortem findings:
Fibrinous inflammation of the intestine (fibrinous enteritis) with bloody mucoid exudate
Whitish blood streaked faeces
(C) E. maxima
E. maxima is found in the middle part of the small intestine and less frequently, throughout the intestine
Postmortem findings:
Haemorrhagic enteritis showing a thickened wall and petechial haemorrhage
Large oocysts on microscopy
(D) E. tenella
E. tenella is found in the ceca. It causes caecal coccidiosis.
Postmortem findings:
Haemorrhage in the ceca (Fig. 209)
In later stages core, of clotted blood and thickened whitish mucosa
In young birds, bloody diarrhoea
(E) E. necatrix
E. necatrix is found in the middle part of the intestine near the yolk sac diverticulum. In severe cases, this coccidium is observed throughout the intestine and ceca. It causes acute mortality and weight loss in laying birds.
Postmortem findings: Intestinal distention, mucoid blood filled exudate and white spots noted on the serosa (Fig. 210)
Fig. 209: Coccidiosis. Haemorrhage in the ceca characteristic of E. tenella infection.
Fig. 210: Intestinal distention, mucoid blood filled exudate and white spots noted on the serosa with E. necatrix infection.
II. Coccidiosis in turkeys
Three species of coccidia in turkeys are considered pathogens. These include E. meleagrimitis, E. adenoeides and E. gallopavonis.
(A) E. meleagrimitis
E. meleagrimitis is found in small intestine. It is the most pathogenic for turkeys.
Antemortem and postmortem findings:
Dehydrated birds showing the breast skin adhering to underlying muscles
Severe inflammation in duodenum and upper jejunum
The upper part of intestine contain excessive fluid and the lower part contain a greenish mucus
(B) E. adenoeides
E. adenoeides is found in the lower small intestine, ceca and large intestine.
Postmortem findings:
Severe inflammation of intestinal mucosa and swollen and edematous intestinal wall
Fluid faeces with mucus and small amount of blood
(C) E. gallopavonis
E. gallopavonis is found in the lower one-third of small intestine, large intestine and ceca.
Postmortem findings:
Necrotic enteritis associated with creamy white exudate
Thickened and damaged intestinal wall devoid of food
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